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KMID : 0359319930330040701
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Korean Journal of Veterinary Research
1993 Volume.33 No. 4 p.701 ~ p.710
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Studies on antitumor effects of pine needles , Pinus densiflora Sieb . et Zucc
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Abstract
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The pine needles, Pinus densiflow Sieb. et Zucc., which is a feed for goats showing a low incidence rate of cancer were evaluated to confirm the potent anticancer effects, with or without several conventional anticancer drugs. The pine needles collected from Mt. Buk-Han located near Seoul were extracted with 95% methanol and methand and concentrated. From the methanol extract, SOM-A, was extracted dichlormethane and SOM-B was extracted with ethyl acetate. SOM-C was extracted with distilled water. These extracts were tested for their antitumor activities in vitro and in vivo. Among them, SOM-A and SOM-C exhibited potent antitumor activities described as belows.
1. The cytotoxic effects of SOM-A and SOM-C were examined against in vitro cultured murine and humman tumor cells.
SOM-A showed strong cytotoxicity against human tumor cell lines and SOM-C showed strong cytotoxicity against murine tumor cell lines tested.
2. The antitumor effects of SOM-A and SOM-C were examined against P388 and L1210 of mouse ascitic tumors.
The highest mean survival time(MST) ration was 151%(P388) for SOM-C(90㎎/㎏).
3. To compare the antitumor effects of SOM-A, SOM-B, and SOM-C against solid tumors, S_180 and Ehrlich carcinoma were implanted subcutaneously to mice on Day O. The drugs were given intraperitoneally to mice once a day on Days 1-20, and the tumor weights were measured on Day 21.
SOM-A showed inhibition of tumor growth more than 50% in the experiment on S_180 and Ehrlich, and SOM-C also markedly inhibited tumor growth. However, SOM-B had no effect.
4. SOM-C combined with α-interferon and SOM-C combined with Mitomycin-C enhanced the antitumor activities against murine ascitic tumors P388 leukemia.
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KEYWORD
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